Dulbecco’s Modified Eagle’s Medium/Nutrient Mixture F-12 Ham

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	supplemented with 10% fetal calf serum (FCS) (Sigma-Aldrich) and 5% Myco Zap (Lonza Verviers SPRL, Verviers, Belgium)

Protocol tips
supplemented with 10% fetal calf serum (FCS) (Sigma-Aldrich) and 5% Myco Zap (Lonza Verviers SPRL, Verviers, Belgium)

Publication protocol

The human pancreatic adenocarcinoma cell lines PaTu 8988t and Panc-1 were obtained from Professor Ellenrieder (Philipps University of Marburg, Germany). The colorectal carcinoma cell line SW 480 was purchased from the German Collection of Microorganism and Cell Culture (DSMZ). PaTu 8988t and Panc-1 cells were maintained in Dulbecco’s modified Eagle’s medium (Sigma-Aldrich, Steinsheim, Germany) or RPMI 1640 (Pan Biotech, Aidenbach, Germany), which was supplemented with 10% fetal calf serum (FCS) (Sigma-Aldrich) and 5% Myco Zap (Lonza Verviers SPRL, Verviers, Belgium) or 5% penicillin plus streptomycin (Sigma-Aldrich) for SW 480. Cells were cultured at 37 °C in humidified CO2 atmosphere (5%) and maintained in monolayer culture. Experiments were done with cells at ~ 70–80% confluence.

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Manufacturer protocol

Download the product protocol from Sigma-Aldrich for Dulbecco’s Modified Eagle’s Medium/Nutrient Mixture F-12 Ham below.

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