Gibco™ RPMI 1640 Medium

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	supplemented with 10% fetal bovine serum (FBS) and 1% penicillin and streptomycin (Invitrogen, Carlsbad, CA, USA)

Protocol tips
supplemented with 10% fetal bovine serum (FBS) and 1% penicillin and streptomycin (Invitrogen, Carlsbad, CA, USA)

Publication protocol

Human prostate cancer cell lines PC3 and DU145 were obtained from the American Type Culture Collection (Manassas, VA, USA). They were maintained in RPMI-1640 supplemented with 10% fetal bovine serum (FBS) and 1% penicillin and streptomycin (Invitrogen, Carlsbad, CA, USA). The paclitaxel-resistant cell lines PC3-TxR and DU145-TxR were kind gifts provided by Professor Atsushi Mizokami (Kanazawa University, Kanazawa, Japan) and were maintained in 10 nmol/L paclitaxel-contained RPMI-1640 medium. Cell morphology was obsevered under an inverted microscope (Olympus, Tokyo, Japan) at 200× magnification. Paclitaxel was purchased from Invitrogen. Antibodies against E-cadherin, Vimentin, Snail, β-catenin, Claudin-1, Notch-1, Notch-2, Notch-4, Akt, glycogen synthase kinase-3β (GSK-3β), phosphorylated GSK-3β (p-GSK-3β), and nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) p65 were purchased from Cell Signaling Technology (Beverly, MA, USA) and β-actin was from Sigma (St. Louis, MO, USA). Human E-cadherin-specific small interfering RNAs (siRNAs), control siRNA, and lentiviral E-cadherin and control vectors were purchased from Genepharma (Shanghai, China). The luciferase reporter lentiviral vector and luciferase-expressing cells were constructed in our own laboratory. The γ-secretase inhibitor (GSI, a Notch inhibitor) was purchased from Calbiochem (San Diego, CA, USA).

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Manufacturer protocol

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