Gibco™ RPMI 1640 Medium

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	Supplement the medium with 10% fetal bovine serum (FBS; Thermo Fisher Scientific, Inc., Waltham, MA, USA)

Protocol tips
Supplement the medium with 10% fetal bovine serum (FBS; Thermo Fisher Scientific, Inc., Waltham, MA, USA)

Publication protocol

The LNCaP cell line, which was derived from a metastatic site of the left supraclavicular lymph node, the DU145 cell line, which was derived from a metastatic site in the brain, and the human immortalized prostatic cell line PNT1A were obtained from American Type Culture Collection (Manassas, VA, USA). The cells were cultured in Gibco RPMI-1640 medium containing 10% fetal bovine serum (FBS; Thermo Fisher Scientific, Inc., Waltham, MA, USA) at 37°C in a humidified atmosphere containing 5% CO2. BCTC and vehicle dimethyl sulfoxide (DMSO) were purchased from Sigma-Aldrich (St. Louis, MO, USA).

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Manufacturer protocol

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