HyClone RPMI 1640 media: Liquid

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	Supplement the medium with 10% fetal bovine serum (FBS) (Hyclone, Waltham, MA, USA), penicillin and streptomycin (0.1 mg·mL−1)

Protocol tips
Supplement the medium with 10% fetal bovine serum (FBS) (Hyclone, Waltham, MA, USA), penicillin and streptomycin (0.1 mg·mL−1)

Publication protocol

The PNT1A (immortalization of normal, adult, prostatic, epithelial cells), PC3 (androgen-independent) and 22RV1 (androgen-dependent) prostatic cancer cell lines were obtained from the American Type Culture Collection (ATCC) (Manassas, VA, USA). The cells were cultured in a complete RPMI-1640 medium (Hyclone, Waltham, MA, USA) with 10% fetal bovine serum (FBS) (Hyclone, Waltham, MA, USA), supplemented with penicillin and streptomycin (0.1 mg·mL−1) at 37 °C and 5% CO2 in a humidified incubator.

Full paper   Login or join for free to view the full paper.

Reviews

Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing Mammalian cell culture media PNT1A using HyClone RPMI 1640 media: Liquid from Cytiva.

Manufacturer protocol

Download the product protocol from Cytiva for HyClone RPMI 1640 media: Liquid below.

Videos

Check out videos that might be relevant for performing Mammalian cell culture media PNT1A using HyClone RPMI 1640 media: Liquid from Cytiva. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.