Dulbecco’s Modified Eagle’s Medium/Nutrient Mixture F-12 Ham

Protocol tips

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	Supplement the medium with 10% FBS.

Protocol tips
Supplement the medium with 10% FBS.

Publication protocol

PhD CX7C phage library was purchased from New England Biolabs (NEB). Fetal calf serum (FCS) and cell culture media (Dulbecco's modified Eagle's medium, DMEM) was purchased from Sigma (USA). The phage display library contains random peptides constructed at the N terminus of the minor coat protein (cpIII) of M13 phage. The library contains a mixture of 3.1 × 109 individual clones, representing repertoire of phages with 7-mer peptide sequences, which expresses random 7-amino-acid sequences. The Escherichia coli host strain ER2738 (F+ strain, New England Biolabs) was used for M13 phage propagation. The human breast cancer cell lines MDA-MB-231, MCF-7 and breast fibroblasts (Hs 578T) were purchased from the American Type Culture Collection. MCF-7 cells were treated 1ng/mL of TGFβ for 16 days. MCF-7, MDA-MB-231, MCF-7/TGFβ, breast cancer cells, and SW620, colon cancer cells, were maintained in DMEM supplemented with 10% fetal bovine serum (Sigma) at 37°C. PC3, prostate cancer cells, were cultured in RPMI1640 media supplemented with 10% FBS at 37°C.Breast fibroblasts (Hs578T) were maintained in special hybricare medium supplemented with 15% FBS (ATCC).

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Manufacturer protocol

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