RPMI 1640, with L-Glutamine and 25mM HEPES

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	supplemented with glutamine and 10% FBS,

Protocol tips
supplemented with glutamine and 10% FBS,

Publication protocol

THP-1 (ATCC TIB-202TM) and HL-60 (ATCC CCL-240TM) cell lines were obtained from the American Cell Culture Collection (ATCC). THP-1 cell are frequently used cells as seen as a model system for monocytes in various studies. They respond with alike transcriptional pattern as peripheral blood mononuclear cells derived macrophages (13-17). In addition, HL-60 cells can differentiate into neutrophils in response to various stimuli like dimethyl sulfoxide, retinoic acid, and dibutyryl cyclic adenosine monophosphate (Bt2 -cAMP). Due to the differentiation potential of these cells, neutrophil differentiated HL-60 cell lines were obtained through incubating them with 1.75% dimethyl sulfoxide
and then were selected as a neutrophil model in this study. The cells were maintained in Roswell Park Memorial Institute (RPMI 1640 (Lonza; Verviers, Belgium) medium supplemented with glutamine and 10% FBS, and grown at 37°C in a 5% CO2 incubator. Cells were grown in six-well tissue culture plates until reaching their desired confluency.

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Manufacturer protocol

Download the product protocol from Lonza for RPMI 1640, with L-Glutamine and 25mM HEPES below.

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