Gibco™ RPMI 1640 Medium

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	supplemented with 10% fetal bovine serum (FBS, PAA) and 10 U/mL penicillin-streptomycin (Gibco)

Protocol tips
supplemented with 10% fetal bovine serum (FBS, PAA) and 10 U/mL penicillin-streptomycin (Gibco)

Publication protocol

The human hematopoietic progenitor tumor cell lines HL60, U937, K562, KG1, and THP-1 were cultured in IMDM (HL60, U937, K562, and KG1) (Invitrogen) or RPMI (THP-1) supplemented with 10% fetal bovine serum (FBS, PAA) and 10 U/mL penicillin-streptomycin (Gibco) at 37°C in a 5% CO2 humidified atmosphere. The Vero cell line was taken along as a positive control cell line and these cells were cultured in Virus Production Serum-Free Medium (VP-SFM, Invitrogen) supplemented with 2 mM glutamine (Life Technologies) at 37°C in a 5% CO2 humidified atmosphere. CHO suspension cells, which served as a negative control, were cultured in Ex-cell 302 medium (Sigma) in shaker flasks (Corning) gently rotating at 50–100 rpm at 37°C in a 5% CO2 humidified atmosphere.

To determine whether K562 cells were capable of growing in bioreactors, Cultibags (Sartorius Stedim) were inoculated with cells at a concentration of 0.3 × 106 cells/mL in 1 L of culture medium. Cultibag cultures were performed at 37°C, dissolved oxygen concentration of 50%, pH 7.2 at a constant rocking speed of 10 rpm, and an angle of 7°.

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Manufacturer protocol

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