DMEM High Glucose w/ L-Glutamine w/o Sodium Pyruvate

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	supplemented with fetal bovine serum 10% (FBS, Biowest, FRA), pyruvate (1 mM; InVitro SA, MEX), non-essential amino acids (0.1 mM; InVitro SA, MEX), and a mix of antibiotics (1 mM; InVitro SA, MEX)

Protocol tips
supplemented with fetal bovine serum 10% (FBS, Biowest, FRA), pyruvate (1 mM; InVitro SA, MEX), non-essential amino acids (0.1 mM; InVitro SA, MEX), and a mix of antibiotics (1 mM; InVitro SA, MEX)

Publication protocol

U87, U251, and D54 cell lines derived from human GBM were used in this study. U87 and U251 cell lines were acquired at ATCC, and D54 cell line was generously obtained by Dr. Andrés Gutiérrez from Dr. Sontheimer's (University of Alabama, Birmingham, AL, USA). Cell lines were cultured with Dulbecco's Modified Eagle Medium with phenol red (DMEM, Biowest, FRA) and supplemented with fetal bovine serum 10% (FBS, Biowest, FRA), pyruvate (1 mM; InVitro SA, MEX), non-essential amino acids (0.1 mM; InVitro SA, MEX), and a mix of antibiotics (1 mM; InVitro SA, MEX). Cells were incubated with CO2 at 5% and at 37°C. Cells were grown until reaching a 70–80% confluence.

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Download the product protocol from Biowest for DMEM High Glucose w/ L-Glutamine w/o Sodium Pyruvate below.

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