DMEM–Dulbecco's Modified Eagle Medium

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	supplemented with 10% (vol/vol) fetal bovine serum (FBS, Gibco)

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supplemented with 10% (vol/vol) fetal bovine serum (FBS, Gibco)

Publication protocol

Human glioma cell lines (A172, U87-MG,
U251, T98G) were obtained from the American
Type Culture Collection (ATCC, Manassas, VA,
USA); all glioma cell lines and 293T cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM; Gibco, Grand Island, NY, USA)
supplemented with 10% (vol/vol) fetal bovine
serum (FBS, Gibco). Human brain microvascular endothelial cells (HBMECs) were purchased
from 3H Biomedical (Stockholm, Sweden) and
cultured in EC medium supplemented with 5%
FBS and EC growth supplement. HBMECs at
passages 2-10 were used in the experiments as
described below. The routine culture was done
in a humidified incubator maintained at 37°C with 5% CO2
and 95% air.

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