DMEM–Dulbecco's Modified Eagle Medium

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	supplemented with penicillin/streptomycin.

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supplemented with penicillin/streptomycin.

Publication protocol

All cells were purchased from the American Type Culture Collection (ATCC). The following cell lines were used in this study. RWPE-1 (ATCC, CRL-11609TM), LNCaP (ATCC, CRL-1740TM), VCaP (ATCC, CRL-2876TM), PC3 (ATCC, CRL-1435TM), DU145 (ATCC, HTB-81TM), 22Rv1 (ATCC, CRL-2505TM). Culture medium was used as follows: Keratinocyte Serum Free Medium (K-SFM) kit (RWPE-1), RPMI1640 medium with 10% FBS, (LNCaP and 22Rv1), DMEM medium with 10% FBS (VCaP), F-12K medium (PC3), Minimal Essential Medium with 10% FBS (DU145). All medium was supplemented with penicillin/streptomycin. All cell lines were maintained in a humidified atmosphere containing 5% CO2 at 37°C.

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