Dulbecco’s Modified Eagle’s Medium (DMEM)

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	supplemented with 10% fetal bovine serum and 2 mM glutamine

Protocol tips
supplemented with 10% fetal bovine serum and 2 mM glutamine

Publication protocol

MG63 (ECACC, human osteoblast-like osteosarcoma cells, catalogue no. 86051601) and VCaP cells (ECACC, human prostate cancer vertebral metastasis, catalogue no. 06020201) were grown in DMEM with 10% fetal bovine serum and 2 mM glutamine at 37°C in 5% CO2 in a humidified incubator. Where serum starvation was required, cells were cultured in phenol red-free DMEM:F12 supplemented with 100× stock of essential amino acids (5 mL per 500 ml medium) and glutamine (final concentration 2 mM).

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