HyClone™ Dulbecco's Modified Eagles Medium

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	containing 10% fetal bovine serum (FBS, SV30087.02, GE Healthcare Life Sciences), 100U/ml penicillin and 100U/ml streptomycin

Protocol tips
containing 10% fetal bovine serum (FBS, SV30087.02, GE Healthcare Life Sciences), 100U/ml penicillin and 100U/ml streptomycin

Publication protocol

Human U251 glioma cells [Obio Technology (Shanghai) Corp., Ltd, Shanghai, China] were cultured in a 37°C, 5% CO2 humidified incubator using Dulbecco’s modified Eagle’s medium (DMEM, SH30022.01, Hyclone, GE Healthcare Life Sciences, Logan, UT, USA) containing 10% fetal bovine serum (FBS, SV30087.02, GE Healthcare Life Sciences), 100U/ml penicillin and 100U/ml streptomycin. Exponentially growing cells, derived after two or three passages, were used for the following experiments: i) U251 cells treated with 50ng/ml GDNF (Merck KGaA, Darmstadt, Germany) for 0, 0.5, 24 or 48h were used to detect the effects of GDNF on the stack area of GA; ii) U251 cells treated with various concentrations of GDNF (0, 25, 50, 75ng/ml) for 48h were used to determine the optimal GDNF concentration.

Full paper   Login or join for free to view the full paper.

Reviews

Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing Mammalian cell culture media U251 using HyClone™ Dulbecco's Modified Eagles Medium from Cytiva.

Manufacturer protocol

Download the product protocol from Cytiva for HyClone™ Dulbecco's Modified Eagles Medium below.

Download manufacturer protocol

Videos

Check out videos that might be relevant for performing Mammalian cell culture media U251 using HyClone™ Dulbecco's Modified Eagles Medium from Cytiva. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.