DMEM/F-12

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	supplemented with 10% fetal bovine serum (Gibco) and 50U/ml penicillin, and 250 μg/ml streptomycin (Corning)

Protocol tips
supplemented with 10% fetal bovine serum (Gibco) and 50U/ml penicillin, and 250 μg/ml streptomycin (Corning)

Publication protocol

Human GBM cell lines U87, U251, LN229, and SNB19 were obtained from the Institute of Biochemistry and Cell Biology, Chinese Academy of Science (Shanghai, China). Green fluorescent proteins (GFP) were transfected into GBM cells by lentivirus infection method. Cells were maintained in Dulbecco’s modified Eagle’s medium-F12 (DMEM/F12, Gibco) supplemented with 10% fetal bovine serum (Gibco) and 50U/ml penicillin, and 250 μg/ml streptomycin (Corning). All cells were cultured in an incubator at 37 °C with 5% CO2.

Neutralized collagen solutions were prepared by mixing buffer solutions of sterile deionized water, 10X PBS (Corning) and 1 mol/L NaOH (Fluka) with the high concentration collagen (Corning) on ice, according to the manufacturer’s instructions44. The final collagen concentration was 6 mg/mL for all the tests.

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