DMEM (Dulbecco's Modified Eagle's medium)

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	containing 10% fetal bovine serum (FBS) and 1% penicillin/streptomycin.

Protocol tips
containing 10% fetal bovine serum (FBS) and 1% penicillin/streptomycin.

Publication protocol

Three cancer cell types were studied in the present study: highly radioresistant U87 glioblastoma cancer cells, radioresistant SkBr3 breast cancer cells and relatively less radioresistant HeLa cervix cancer cells. U87 and HeLa cells were obtained from ATCC (American Type Culture Collection, Manassas, VA, USA). SkBr3 was commercially available and used for several SMLM studies in our laboratory. U87 and HeLa cells were grown in Dulbecco’s modified essential medium (Thermo Fisher Scientific, Waltham, MA, USA) supplemented with 10% heat-inactivated fetal calf serum (Thermo Fisher Scientific, Waltham, MA, USA), 100 U/mL penicillin (PAA), 100 μg/mL streptomycin (PAA), and 1% NEAA (Thermo Fisher Scientific, Waltham, MA, USA). Cell cultures were kept in T-25 cell flasks at 37 °C in a humidified atmosphere with 5% CO2.

For the experiments with SMLM, SkBr3 cells were prepared as described. SkBr3 cells were grown in McCoy’s 5A cell medium, containing 10% fetal bovine serum (FBS) and 1% penicillin/streptomycin. Cells were cultivated and maintained at 37 °C in a humidified atmosphere at 95% air/5% CO2. Then, the cells were trypsinized and transferred to coverslips, put into six-well plates and further cultivated (about three passages, i.e., about 38 h) until 80% confluence.

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