Corning™ Basal Cell Culture Liquid Media - DMEM and Ham's F-12, 50/50 Mix

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	supplemented with 10% FBS and 1% penicillin–streptomycin–amphotericin,

Protocol tips
supplemented with 10% FBS and 1% penicillin–streptomycin–amphotericin,

Publication protocol

A normal HSG cell line was received as a generous gift (Dr. M. Hoffman, National Institutes of Health [NIH], Bethesda, MA). A submaxillary salivary gland cancer cell line, HTB-41, was acquired from the American Type Culture Collection (ATCC; Manassas, VA). HSG and HTB-41 cells were aseptically cultured in DMEM/F-12 and McCoys5A media, respectively (Corning Cellgro; Manassas, VA), supplemented with 10% FBS and 1% penicillin–streptomycin–amphotericin, in 5% CO2 atmosphere at 37C. All cell culture experiments were carried out in triplicate.

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