Dulbecco’s Modified Eagle’s Medium (DMEM)

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	supplemented with 10% Fetal Bovine Serum, FBS (Gemini Bioproducts).

Protocol tips
supplemented with 10% Fetal Bovine Serum, FBS (Gemini Bioproducts).

Publication protocol

293T, Jurkat, KG-1, Cem-A, Molt and Cem-SS cells were obtained from the American Type Culture Collection (ATCC). 293T cells were maintained in Dulbecco’s Modified Eagle Medium (Cellgro) supplemented with 10% Fetal Bovine Serum, FBS (Gemini Bioproducts). Rest of the cells were maintained in Iscove's Modified Dulbecco's Medium (ATCC) supplemented with 20% FBS.

The following reagents were obtained through the AIDS Research and Reference Reagent Program, Division of AIDS, NIAID, NIH; p24 Monoclonal Antibody (183-H12-5C) from Dr. Bruce Chesebro and Kathy Wehrly, pSV-Ψ-MLV-env- from Dr. Nathaniel Landau.

Goat-anti-mouse-horseradish peroxidase and goat-anti-rabbit-horseradish peroxidase (HRP) secondary antibodies and West Femto enhanced chemiluminescent (ECL) HRP substrate were obtained from Thermo Scientific (Rockford, IL). Rabbit polyclonal Antibody against gp96 was obtained from GeneTex (Irvine, CA). Mouse monoclonal antibody against GAPDH was obtained from ABM (Richmond, BC, Canada). VSVG mouse monoclonal antibody was obtained from KeraFAST. DiI-LDL was obtained from Kalen Biochemical (Montgomery Village, MD) VSV-eGFP was a kind gift from Dr. Asit Pattnaik (University of Nebraska-Lincoln). Measles-GFP was a kind gift from Dr. Stephen Russell (Mayo Clinic, Rochester).

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Manufacturer protocol

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