DMEM–Dulbecco's Modified Eagle Medium

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	supplemented with 10% fetal bovine serum (both from Gibco; Thermo Fisher Scientific, Inc., Waltham, MA, USA)

Protocol tips
supplemented with 10% fetal bovine serum (both from Gibco; Thermo Fisher Scientific, Inc., Waltham, MA, USA)

Publication protocol

293-T and Cos-7 (originating from African green monkey kidney fibroblasts) cell lines (Cell Bank of the Chinese Academy of Sciences, Shanghai, China) were cultured in Dulbecco's modified Eagle's medium (DMEM) supplemented with 10% fetal bovine serum (both from Gibco; Thermo Fisher Scientific, Inc., Waltham, MA, USA), and incubated at 37°C incubator in an atmosphere containing 5% CO2. Cells were passaged at 80% confluency by digestion with trypsin (Gibco; Thermo Fisher Scientific, Inc.).

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Manufacturer protocol

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