DMEM–Dulbecco's Modified Eagle Medium

Mammalian cell culture media 293T

Experiment
Mammalian cell culture media 293T
Product
DMEM–Dulbecco's Modified Eagle Medium from Thermo Fisher Scientific
Manufacturer
Thermo Fisher Scientific

Protocol tips

Protocol tips
supplemented with 10% fetal bovine serum (both from Gibco; Thermo Fisher Scientific, Inc., Waltham, MA, USA)

Publication protocol

293-T and Cos-7 (originating from African green monkey kidney fibroblasts) cell lines (Cell Bank of the Chinese Academy of Sciences, Shanghai, China) were cultured in Dulbecco's modified Eagle's medium (DMEM) supplemented with 10% fetal bovine serum (both from Gibco; Thermo Fisher Scientific, Inc., Waltham, MA, USA), and incubated at 37°C incubator in an atmosphere containing 5% CO2. Cells were passaged at 80% confluency by digestion with trypsin (Gibco; Thermo Fisher Scientific, Inc.).

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Papers

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Paper title
A novel splice mutation induces exon skipping of the EXT1 gene in patients with hereditary multiple exostoses
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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for DMEM–Dulbecco's Modified Eagle Medium below.

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