Modified IMEM (Improved Minimum Essential Medium)

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	Supplement the medium with 10% FBS.

Protocol tips
Supplement the medium with 10% FBS.

Publication protocol

Human medulloblastoma cell line Daoy, provided by the American Type Culture Collection (ATCC, Manassas, VA, USA) was incubated in improved minimum essential medium (IMEM, Invitrogen, Carlsbad, CA, USA) containing 10% fetal bovine serum (FBS, Sigma-Aldrich, St. Louis, MO, USA) (37°C, 5% CO2). Daoy cells were treated by LSZ (W323713, natural, ≥98%, Sigma-Aldrich) for 24 h through incubating cells in culture medium added with water-LSZ solution.

Full paper   Login or join for free to view the full paper.

Reviews

Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing Mammalian cell culture media DAOY using Modified IMEM (Improved Minimum Essential Medium) from Thermo Fisher Scientific.

Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for Modified IMEM (Improved Minimum Essential Medium) below.

Download manufacturer protocol

Videos

Check out videos that might be relevant for performing Mammalian cell culture media DAOY using Modified IMEM (Improved Minimum Essential Medium) from Thermo Fisher Scientific. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.