Gibco™ MEM α, Nucleosides

Protocol tips

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	supplemented with 2 mM L-glutamine, 0.2 mM myo-inositol, 0.02 mM folic acid, 0.1 mM 2-mercaptoethanol, 400 IU/ml IL-2 (PeproTech, America), 12.5% FBS, 12.5% horse serum (Gibco, Life Technologies, America), and 1% penicillin/streptomycin

Protocol tips
supplemented with 2 mM L-glutamine, 0.2 mM myo-inositol, 0.02 mM folic acid, 0.1 mM 2-mercaptoethanol, 400 IU/ml IL-2 (PeproTech, America), 12.5% FBS, 12.5% horse serum (Gibco, Life Technologies, America), and 1% penicillin/streptomycin

Publication protocol

Human colorectal cancer cell lines (HCT116 and HCT-8) as well as the human embryonic kidney epithelial cell line 293T were cultured in Dulbecco's modified Eagle's medium (DMEM, Gibco, Life Technologies, America). The human colonic epithelial cell line FHC was cultured in RPMI-1640 medium (Gibco, Life Technologies, America). The human colorectal cancer cell line SW620 was cultured in Leibovitz's L-15 medium (Gibco, Life Technologies, America). All the above media were supplemented with 10% FBS and 1% penicillin/streptomycin (Gibco, Life Technologies, America). NK-92, CAR-NK-92, and Ctrl-NK-92 cells were incubated in alpha modification of Eagle's minimum essential medium (α-MEM, Gibco, Life Technologies, America) supplemented with 2 mM L-glutamine, 0.2 mM myo-inositol, 0.02 mM folic acid, 0.1 mM 2-mercaptoethanol, 400 IU/ml IL-2 (PeproTech, America), 12.5% FBS, 12.5% horse serum (Gibco, Life Technologies, America), and 1% penicillin/streptomycin. All cell lines were cultured at 37°C in a humidified atmosphere with 5% CO2.

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