Leibovitz's L-15 Medium

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	supplemented with 10% FBS (Thermo Fisher Scientific, Waltham, MA, USA).

Protocol tips
supplemented with 10% FBS (Thermo Fisher Scientific, Waltham, MA, USA).

Publication protocol

Four breast cancer cell lines (MDA-MB-231, MDA-MB-468, BT-549 and MCF-7) were obtained from the Cell Bank of Chinese Academy of Sciences (Shanghai, China). MDA-MB-231 and MCF-7 cell lines were cultured in DMEM (Life Technologies) and incubated at 37°C with 5% CO2. The BT-549 cell lines were cultured in RPMI 1640 (Life Technologies) and incubated at 37°C with 5% CO2. The MDA-MB-468 cell line was cultured in L15 (Life Technologies) at 37°C without CO2. All media were supplemented with 10% FBS (Thermo Fisher Scientific, Waltham, MA, USA).

Full paper   Login or join for free to view the full paper.

Reviews

Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing Mammalian cell culture media MDA-MB-468 using Leibovitz's L-15 Medium from Thermo Fisher Scientific.

Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for Leibovitz's L-15 Medium below.

Download manufacturer protocol

Videos

Check out videos that might be relevant for performing Mammalian cell culture media MDA-MB-468 using Leibovitz's L-15 Medium from Thermo Fisher Scientific. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.