IMDM

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	supplemented with 10% fetal bovine serum (FBS, PAA) and 10 U/mL penicillin-streptomycin (Gibco)

Protocol tips
supplemented with 10% fetal bovine serum (FBS, PAA) and 10 U/mL penicillin-streptomycin (Gibco)

Publication protocol

The human hematopoietic progenitor tumor cell lines HL60, U937, K562, KG1, and THP-1 were cultured in IMDM (HL60, U937, K562, and KG1) (Invitrogen) or RPMI (THP-1) supplemented with 10% fetal bovine serum (FBS, PAA) and 10 U/mL penicillin-streptomycin (Gibco) at 37°C in a 5% CO2 humidified atmosphere. The Vero cell line was taken along as a positive control cell line and these cells were cultured in Virus Production Serum-Free Medium (VP-SFM, Invitrogen) supplemented with 2 mM glutamine (Life Technologies) at 37°C in a 5% CO2 humidified atmosphere. CHO suspension cells, which served as a negative control, were cultured in Ex-cell 302 medium (Sigma) in shaker flasks (Corning) gently rotating at 50–100 rpm at 37°C in a 5% CO2 humidified atmosphere.

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