Gibco™ RPMI 1640 Medium

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	supplemented with 10% heat-inactivated fetal calf serum (FCS), 1% L-glutamine, and a 1% antibiotic mixture of penicillin, streptomycin, and neomycin (PSN), all from GIBCO-BRL (Gaithersburg, MD).

Protocol tips
supplemented with 10% heat-inactivated fetal calf serum (FCS), 1% L-glutamine, and a 1% antibiotic mixture of penicillin, streptomycin, and neomycin (PSN), all from GIBCO-BRL (Gaithersburg, MD).

Publication protocol

KG 1 and KG 1 a were kindly provided by Dr Mannoni (Marseille, France). HL60 cells were purchased from the Human Mutant Cell Repository Institute for Medical Research (Camden, NJ). All these cells were maintained at 37”C, in a fully humidified atmosphere of 5% CO2 in air, in RPM1 1640 culture medium supplemented with 10% heat-inactivated fetal calf serum (FCS), 1% L-glutamine, and a 1% antibiotic mixture of penicillin, streptomycin, and neomycin (PSN), all from GIBCO-BRL (Gaithersburg, MD). They were subcultured twice a week and studied in
the exponential growth phase.

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Manufacturer protocol

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