RPMI-1640 with Phenol Red produced by FUJIFILM Wako Pure Chemical Corporation

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	supplemented with 10% FBS, 100 units/mL penicillin, and 100 μg/mL streptomycin

Protocol tips
supplemented with 10% FBS, 100 units/mL penicillin, and 100 μg/mL streptomycin

Publication protocol

Human basophil KU812 cells obtained from Health Science Research Resources Bank (Osaka, Japan) were maintained in RPMI-1640 medium containing 10% FBS, 100 units/mL penicillin, and 100 μg/mL streptomycin, under 5% CO2 at 37 °C. After pre-cultured KU812 cells with RGE or ginsenosides (Rb1, Rg1, Rg3, and Rh1) for 12 h, cells were changed all of free fresh media and were stimulated with an anti-FcεRIα antibody (0.02% serum antibody against human FcεRIα) in the absence or presence of RGE, compared with control serum antibody from non-immune rabbit. KU812 cells were washed with phosphate-buffered saline and collected by centrifugation in ice-cold phosphate-buffered saline.

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