ElectraSense 4x2K/12K

Protocol tips

Upstream tips	Protocol tips	Downstream tips
the Re-usable plateform can be re-hybridized up to 3 times after 1st usage but need to strip first

Upstream tips
the Re-usable plateform can be re-hybridized up to 3 times after 1st usage but need to strip first

Publication protocol

Microarray analysis of the biotin-labeled DNA samples was accomplished according to the manufacturer's instructions: ElectraSense 4x2K/12K Microarrays: Hybridization and Electrochemical Detection manual (CustomArray, Inc.). All reagent preparations and procedures were followed except for a modification to the hybridization step: 13.5 μL of purified biotinylated DNA was combined with 16.5 μL of the hybridization solution. The hybridization solution was also prepared without the addition of nuclease-free water to account for the additional sample volume. Hybridization was conducted using a UVP HB-500 Minidizer hybridization oven (Ultra-Violet Products, LLC, Upland, CA) that was modified to hold the microarrays in microarray clamps fixed onto a rotisserie wheel. Hybridization was optimized with an incubation time of 2 h at 50°C with rotation. ElectraSense Detection kits were used for post-hybridization blocking, labeling, washing, and ECD according to the manufacturer's instructions (CustomArray, Inc.).

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Manufacturer protocol

Download the product protocol from Custom Array for ElectraSense 4x2K/12K below.

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