Keratinocyte-SFM

Protocol tips

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	Refresh the medium every two days and sub culture after every four days.

Protocol tips
Refresh the medium every two days and sub culture after every four days.

Publication protocol

HaCaT cells, a spontaneously transformed aneuploid immortal keratinocyte cell line from adult human skin, were purchased from Cells Center of Shanghai Institutes for Biological Sciences (Chinese Academy of Science, Shanghai, China), cultured in defined keratinocyte serum-free medium (K-SFM) (Gibico, Grand Island, NY, USA), and grown at 37 °C in a humidified incubator in a 5% CO2 atmosphere. The medium was refreshed every two days, and cells were sub-cultured every four days. Cell culture was performed according to the manufacturer’s manual.

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Manufacturer protocol

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