Dulbecco’s Modified Eagle’s Medium (DMEM)

Mammalian cell culture media HepG2

Experiment
Mammalian cell culture media HepG2
Product
Dulbecco’s Modified Eagle’s Medium (DMEM) from Welgene
Manufacturer
Welgene

Protocol tips

Protocol tips
supplemented with 1% sodium pyruvate (Sigma), 1.5 g/L sodium bicarbonate, 10% fetal bovine serum and 1% antibiotics, including 1% penicillin/streptomycin and 25 ug/ml amphotericin B (Sigma) at 37 °C in 5% CO2

Publication protocol

The Hep G2 and LCL-PI 11 cells were cultured with DMEM (pH 7.2–7.4) supplemented with 1% sodium pyruvate (Sigma), 1.5 g/L sodium bicarbonate, 10% fetal bovine serum and 1% antibiotics, including 1% penicillin/streptomycin and 25 ug/ml amphotericin B (Sigma) at 37 °C in 5% CO2 to promote attachment. When the cells reached > 80% confluence, 5 × 105 cells were seeded into 96-well plates, allowed to adhere for 24 h and subsequently treated with medium containing different doses of E2 (0.01, 0.1, 1, 5 and 10 μM/lit) to determine the viability and IC50 values. After 24, 48, 72 and 96 h of the treatment, the effect of E2 was assessed by MTT assay according to standard protocols. In this regard, the Hep G2 and LCL-PI 11 cells were washed twice with PBS, and a fresh medium containing MTT (0.5 mg/mL) was added and finally, after 4-hour incubation, the formazan crystals were dissolved in DMSO and the absorbance was measured at 570 nm. All experiments were repeated three times, with at least three measurements (triplicates).

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Papers

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Paper title
Comparative Analysis of the Effects of 17-Beta Estradiol on Proliferation, and Apoptosis in Hepatocellular Carcinoma Hep G2 and LCL-PI 11 Cell Lines
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Manufacturer protocol

Download the product protocol from Welgene for Dulbecco’s Modified Eagle’s Medium (DMEM) below.

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