RPMI-1640 Medium

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	supplemented with 10% fetal bovine serum, penicillin G (100 U/mL), and streptomycin sulfate (100 μg/mL)

Protocol tips
supplemented with 10% fetal bovine serum, penicillin G (100 U/mL), and streptomycin sulfate (100 μg/mL)

Publication protocol

The HL-60 human promyelocytic leukemia cells were routinely maintained under a 5% CO2 atmosphere at 37°C in RPMI 1640 medium supplemented with 10% fetal bovine serum, penicillin G (100 U/mL), and streptomycin sulfate (100 μg/mL). For the cell proliferation assay, HL-60 cells were seeded in individual wells of a 96-well culture plate at a density of 2 × 104 cells/100 μL per well in the same medium. The cell counting kit-8 was used to measure the activities of dehydrogenase enzyme(s) in viable cells according to the manufacturer's instructions. Briefly, 1 μL of cheese extracts, reconstituted in DMSO, was added to each well. After incubation for 24 h, 10 μL of 5 mM WST-8 solution containing 0.2 mM 1-methoxy-5-methyl-phenazinium-methyl-sulfate and 150 mM NaCl was added to each well, followed by another 3 h of incubation. The reduction of WST-8 was determined colorimetrically at 450 nm using an automatic microplate spectrophotometer (model 550, BioRad Laboratories Inc., Hercules, CA).

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Manufacturer protocol

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