HyClone RPMI 1640 media: Liquid

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	RPMI-1640 culture medium containing 10% fetal bovine serum (FBS), 100 U/ml penicillin, and 100 U/ml streptomycin and were continuously cultured in an incubator with saturated humidity (37°C and 5% CO2)

Protocol tips
RPMI-1640 culture medium containing 10% fetal bovine serum (FBS), 100 U/ml penicillin, and 100 U/ml streptomycin and were continuously cultured in an incubator with saturated humidity (37°C and 5% CO2)

Publication protocol

K562 cells were maintained in RPMI-1640 culture medium containing 10% fetal bovine serum (FBS), 100 U/ml penicillin, and 100 U/ml streptomycin and were continuously cultured in an incubator with saturated humidity (37°C and 5% CO2). The medium was refreshed every other day. Cells at the logarithmic growth phase were used for experiments. Transfection was performed using the Lipofectamine 2000 transfection reagent according to the instruction manual. The experimental cells were divided into the miR-20a ASODN group, scrambled oligonucleotides (SODNs) group, simple liposome group (blank transfection group), and cell group (the blank group).

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