Anti-LC3A/B antibody (ab62721)

Protocol tips

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	Rat liver tissue was fixed with 4% formaldehyde followed by routine dehydration and paraffin embedding. Paraffinembedded tissue was sliced into 4 um sections, followed by dewaxing with xylene and gradient alcohol hydration in strict accordance with the kit instructions (PV-900, Beijing Zhongyu Jinqiao Biotechnology Co., Ltd.). Beclin1 and LC3 antibodies were purchased from Abcam Corporation, UK, and used at 1:1000 dilution. For negative controls, the primary antibodies were replaced with PBS. The results showed that Beclin1 and LC3 were present in both the cytoplasm and nucleus, the positive expression were light yellow to yellowish brown granules , and the areas with brownish yellow color were positive. Five fields were randomly selected for each section (magnification 400X), 100 cells were counted and the percentage of positive cells was calculated to indirectly estimate the expression levels.

Protocol tips

Rat liver tissue was fixed with 4% formaldehyde followed by routine dehydration and paraffin embedding. Paraffinembedded tissue was sliced into 4 um sections, followed by dewaxing with xylene and gradient alcohol hydration in strict accordance with the kit instructions (PV-900, Beijing Zhongyu Jinqiao Biotechnology Co., Ltd.). Beclin1 and LC3 antibodies were purchased from Abcam Corporation, UK, and used at 1:1000 dilution. For negative controls, the primary antibodies were replaced with PBS. The results showed that Beclin1 and LC3 were present in both the cytoplasm and nucleus, the positive expression were light yellow to yellowish brown granules , and the areas with brownish yellow color were positive. Five fields were randomly selected for each section (magnification 400X), 100 cells were counted and the percentage of positive cells was calculated to indirectly estimate the expression levels.

Publication protocol

Rat liver tissue was fixed with 4% formaldehyde followed by routine dehydration and paraffin embedding. Paraffinembedded tissue was sliced into 4 um sections, followed by dewaxing with xylene and gradient alcohol hydration in strict accordance with the kit instructions (PV-900, Beijing Zhongyu Jinqiao Biotechnology Co., Ltd.). Beclin1 and LC3 antibodies were purchased from Abcam Corporation, UK, and used at 1:1000 dilution. For negative controls, the primary antibodies were replaced with PBS. The results showed that Beclin1 and LC3 were present in both the cytoplasm and nucleus, the positive expression were light yellow to yellowish brown granules , and the areas with brownish yellow color were positive. Five fields were randomly selected for each section (magnification 400X), 100 cells were counted and the percentage of positive cells was calculated to indirectly estimate the expression levels.

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Manufacturer protocol

Download the product protocol from Abcam for Anti-LC3A/B antibody (ab62721) below.

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