Global DNA Methylation ELISA

Protocol tips

Upstream tips	Protocol tips	Downstream tips
Ensure DNA is mixed thoroughly prior to application. The wells of the assay strip bind up to 200 ng of total DNA.

Upstream tips
Ensure DNA is mixed thoroughly prior to application. The wells of the assay strip bind up to 200 ng of total DNA.

Publication protocol

2.8. Quantification of DNA Methylation
The extracted DNA from each sample was used to quantify the global DNA methylation using Global DNA Methylation ELISA Kit (Cell Biolabs Inc., USA). Briefly, a standard curve was initially generated and the kit's instruction was followed. The OD was read at 450 nm using plate reader.



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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing DNA methylation profiling Whole genome profiling - HCT116, HTC15 human colon cancer cells using Global DNA Methylation ELISA from Cell Biolabs.

Manufacturer protocol

Download the product protocol from Cell Biolabs for Global DNA Methylation ELISA below.

Download manufacturer protocol

Videos

Check out videos that might be relevant for performing DNA methylation profiling Whole genome profiling - HCT116, HTC15 human colon cancer cells using Global DNA Methylation ELISA from Cell Biolabs. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.