Anti-RFP antibody

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	- Stain cells with Primary Ab at dilution-1:200 and incubate overnight at 4C.

Protocol tips
- Stain cells with Primary Ab at dilution-1:200 and incubate overnight at 4C.

Publication protocol

For immunohistochemistry in vitro, GFP-LC3/CCL149 cells growing on glass cover slips were fixed for 15min with 4% paraformaldehyde. After being incubated with 0.5% Triton X-100/PBS solution for 30 min and washed with PBS three times, the GFP-LC3/CCL149 cells were blocked with 3% hydrogen peroxide for 15 min and subsequently incubated overnight at 4 °C with the primary antibodies. Binding was visualized with the appropriate peroxidase-conjugated secondary antibodies (AR1022, ZSGB-BIO) for 20-30 min at 37 °C.


Full paper   Login or join for free to view the full paper.

Reviews

Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing Autophagy assay cell type - L2/CCL149 using Anti-RFP antibody from Abcam.

Manufacturer protocol

Download the product protocol from Abcam for Anti-RFP antibody below.

Download manufacturer protocol

Videos

Check out videos that might be relevant for performing Autophagy assay cell type - L2/CCL149 using Anti-RFP antibody from Abcam. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.