Autophagy Inhibitor, 3-MA

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	- Dissolve the inhibitor in distilled water

Protocol tips
- Dissolve the inhibitor in distilled water

Publication protocol

INS-1E cells grown on glass coverslips were fixed for 5 min in 4% ice-cold paraformaldehyde, rinsed in phosphate buffered saline (PBS) and permeabilized for 30 min in PBS supplemented with 1.5% BSA and 0.2% Triton X-100. Coverslips were incubated overnight at 4 °C in the presence of antibody against LC3B and p62 (dilution 1/100; see Table S2). Cells were then washed and further exposed for 1 h to an appropriate Alexa fluor 488-conjugated or 555-conjugated antibodies (1/1000; N.V. Life Technology AG, Switzerland), and photographed under fluorescence microscopy (Leica Camera AG, Nidau, Switzerland).

Full paper   Login or join for free to view the full paper.

Reviews

Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing Autophagy assay cell type - INS-1E using Autophagy Inhibitor, 3-MA from Sigma-Aldrich.

Manufacturer protocol

Download the product protocol from Sigma-Aldrich for Autophagy Inhibitor, 3-MA below.

Download manufacturer protocol

Videos

Check out videos that might be relevant for performing Autophagy assay cell type - INS-1E using Autophagy Inhibitor, 3-MA from Sigma-Aldrich. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.