Beclin-1 Antibody

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	- Before adding primary antibody, incubate membrane with 5% non-fat milk for 1 h at room temperature. - Incubate primary antibody overnight at 4°C

Protocol tips
- Before adding primary antibody, incubate membrane with 5% non-fat milk for 1 h at room temperature. - Incubate primary antibody overnight at 4°C

Publication protocol

Cells were washed once with ice-cold PBS and harvested in a cell lysis buffer mixed with a protease inhibitor cocktail following various treatments. To prepare protein samples for immunoblot, the kidney tissue samples were homogenized with cell lysis buffer and with protease inhibitor cocktail. Proteins were separated by SDS/PAGE and transferred to nitrocellulose membranes. After incubation with 5% non-fat milk for 1 h at room temperature, membranes were incubated with a primary antibody overnight at 4°C and then incubated with appropriate horseradish peroxidase–conjugated secondary antibody for 1 h at room temperature. Bound antibodies were visualized by chemiluminescence detection. The densitometry analysis of immunoblot results was determined by NIH Image software (National Institutes of Health, Bethesda, MD).

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Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing Autophagy assay cell type - NRK-49F using Beclin-1 Antibody from Cell Signaling Technology.

Manufacturer protocol

Download the product protocol from Cell Signaling Technology for Beclin-1 Antibody below.

Download manufacturer protocol

Videos

Check out videos that might be relevant for performing Autophagy assay cell type - NRK-49F using Beclin-1 Antibody from Cell Signaling Technology. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.