Beclin-1 (D40C5) Rabbit mAb

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	- Dilute Primary antibody at 1:1000 and incubate at overnight at 4°C.

Protocol tips
- Dilute Primary antibody at 1:1000 and incubate at overnight at 4°C.

Publication protocol

Western blot analysis was conducted as described previously. Briefly, the cells were lysed in RIPA buffer, and equal amounts of protein were separated on an SDS polyacrylamide gel, transferred to an NC membrane (Millipore) and then immunoblotted with antibodies. Primary antibodies against the following proteins were used in this study: Beclin1(#3495), SQSTM1/P62 (#5114), LC3A/B (#12741), phospho-p38 (T180/Y182, #4511) and p38 (#9212) were purchased from Cell Signaling Technology, 8-OHdG (ab10802) and SOD1 (ab51254) were purchased from Abcam, GAPDH (sc-365062) was purchased from Santa Cruz. The following secondary antibodies used in the study were purchased from Santa Cruz: HRP-conjugated anti-mouse and anti-rabbit IgG (sc-2005 and sc-2004). The band intensities were quantified and normalized to the band intensities of GAPDH using ImageJ software. The data were presented as bar graphs after their statistical validity was tested.

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Manufacturer protocol

Download the product protocol from Cell Signaling Technology for Beclin-1 (D40C5) Rabbit mAb below.

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