EpiQuik Dnmt3A Assay Kit

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	Quantification of nuclear DNMT3A and DNMT3B enzyme concentrations were performed as per the manufacturer's guidelines (Epiquik DNMT3A/B Assay Kit, Epigentek, New York). All samples were assayed in duplicate, and various dilutions of the respective DNMT standards were used to plot a standard curve. Microplates were scanned at 450 nm with a reference wavelength of 655 nm. Intra‐assay coefficient of variations for DNMT3A and DNMT3B were calculated as 3.95% and 9.92%, respectively.

Protocol tips

Quantification of nuclear DNMT3A and DNMT3B enzyme concentrations were performed as per the manufacturer's guidelines (Epiquik DNMT3A/B Assay Kit, Epigentek, New York). All samples were assayed in duplicate, and various dilutions of the respective DNMT standards were used to plot a standard curve. Microplates were scanned at 450 nm with a reference wavelength of 655 nm. Intra‐assay coefficient of variations for DNMT3A and DNMT3B were calculated as 3.95% and 9.92%, respectively.

Publication protocol

DNMT quantification
Quantification of nuclear DNMT3A and DNMT3B enzyme concentrations were performed as per the manufacturer's guidelines (Epiquik DNMT3A/B Assay Kit, Epigentek, New York). All samples were assayed in duplicate, and various dilutions of the respective DNMT standards were used to plot a standard curve. Microplates were scanned at 450 nm with a reference wavelength of 655 nm. Intra‐assay coefficient of variations for DNMT3A and DNMT3B were calculated as 3.95% and 9.92%, respectively.

The following equation was used to quantify enzyme concentration:

Concentration(ng · mg of protein-1) = (sample OD - blank OD)/(slope × protein amount) × 1000
where OD is optical density/absorbance. Slope is generated from the standard curve. Protein amount (μg) as quantified by Bradford assay.

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Manufacturer protocol

Download the product protocol from Epigentek for EpiQuik Dnmt3A Assay Kit below.

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