Anti-LC3 mAb

Protocol tips

Upstream tips	Protocol tips	Downstream tips
- Storage buffer conditions:1 mg/mL in PBS/50% glycerol, pH 7.2

Upstream tips
- Storage buffer conditions:1 mg/mL in PBS/50% glycerol, pH 7.2

Publication protocol

C6 glioma cells were harvested and lysed in modified RIPA buffer (50 mM Tris-HCl, pH 7.4, 1% NP-40, 0.25% sodium deoxycholate, 150 mM NaCl, 1 mM EDTA, 2 mM diisopropylfluorophosphate, 10 µg/ mL of leupeptin, 10 µg/mL of aprotinin, and 10 µg/mL of pepstatin). Cell lysates were centrifuged and proteins in the supernatants were electrophoresed in sodium dodecyl sulfate-polyacrylamide gels and transferred to Immobilon P membranes (Millipore, Billerica, MA). The membranes were blocked with 1% skim milk in 0.1% Tween 20/ PBS (PBS-T) and incubated with primary antibodies. Horseradish peroxidase-conjugated secondary antibodies were used and immunoreactive proteins were detected with ImmunoStar LD (Wako Pure Chemical Industries, Osaka, Japan).

Full paper   Login or join for free to view the full paper.

Reviews

Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing Autophagy assay cell type - C6 using Anti-LC3 mAb from MBL international corporation.

Manufacturer protocol

Download the product protocol from MBL international corporation for Anti-LC3 mAb below.

Download manufacturer protocol

Videos

Check out videos that might be relevant for performing Autophagy assay cell type - C6 using Anti-LC3 mAb from MBL international corporation. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.