LAMP2 (D5C2P) Rabbit mAb

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	- Dilute primary Ab at 1:200.

Protocol tips
- Dilute primary Ab at 1:200.

Publication protocol

GH3 cells grown on culture slides (BD Falcon Labware, REF 354108) were permeabilized and fixed in methanol at −20°C for 3 min. Cells were washed with phosphate-buffered saline (PBS), blocked with 10% bovine serum albumin (Sigma) with PBS for 10 min, and incubated with primary antibody in blocking buffer for 1 h at room temperature (RT). Cells were hybridized with secondary antibodies for 1 h at RT. The coverslips were mounted on glass slides using Vectashield mounting medium (Vector Labs Inc., Burlingame, CA, USA). Cells were viewed under a Leica TCS SP5 confocal microscope (Leica, Microsystems CMS GmbH, Germany). The following primary antibodies were used: Lamp2 (Cell Signaling Technology) and LC3 (Cell Signaling Technology). The following secondary antibodies were used: Alexa 594 (red)-conjugated anti-rabbit IgG (Vector Laboratories Inc.) and fluorescein isothiocyanate (green)-labeled anti-mouse IgG (Jackson ImmunoResearch Laboratories, West Grove, PA, USA).

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Manufacturer protocol

Download the product protocol from Cell Signaling Technology for LAMP2 (D5C2P) Rabbit mAb below.

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