Endothelial Cell Growth Medium MV

Protocol tips

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	supplemented with 5% fetal calf serum and 1% antibiotics (10000 U Penicillin/ml and 10000 U Streptomycin/ml, Lonza)

Protocol tips
supplemented with 5% fetal calf serum and 1% antibiotics (10000 U Penicillin/ml and 10000 U Streptomycin/ml, Lonza)

Publication protocol

The freshly isolated Aortic microvascular ECs (from the adventitia (AECs) and aortic macrovascular ECs from the intima (IECs)) cells were seeded together with the matrix debris generated by the action of the collagenase on non-coated plastic dishes and maintained in EGM-MV (containing endothelial cell growth complement, EGF 10 ng/ml, heparin 90 μg/ml and hydrocortisone 1 μg/ml) supplemented with 5% fetal calf serum and 1% antibiotics (10000 U Penicillin/ml and 10000 U Streptomycin/ml, Lonza). This medium was developed by PromoCell for culturing human ECs from either microvascular or macrovascular origin. HUVEC cells were purchased from Lonza (# cc-2817) and cultured in EGM2-MV medium (Lonza)

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Manufacturer protocol

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