Minimum Essential Medium Eagle

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	All vascular cells were cultured on plates or coverslips coated with 0.2% porcine gelatin (Sigma)

Protocol tips
All vascular cells were cultured on plates or coverslips coated with 0.2% porcine gelatin (Sigma)

Publication protocol

A7r5 rat smooth muscle cells were obtained from the ATCC (Manassas, VA). Bovine aortic endothelial cells (BAOECs), bovine aortic smooth muscle cells (BAOSMCs), and rat aortic smooth muscle cells were obtained from Cell Applications, Inc. (San Diego, CA). Commercially available cells were grown as recommended by the supplier and exchanged into minimum Eagle's medium over time before experiments. Human brain microvascular endothelial cells were obtained from Cell Systems (Kirkland, WA) and cultured using Cell Systems complete medium before exchange into minimum Eagle's medium supplemented identically as the BAOEC culture. All vascular cells were cultured on plates or coverslips coated with 0.2% porcine gelatin (Sigma). Hybridomas were cultured and the monoclonal antibodies were purified using affinity chromatography.





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