Medium 231

Protocol tips

Upstream tips	Protocol tips	Downstream tips
Following Ad pre-treatment, cells were exposed to normoxia (5% CO2 – 95% air) or hyperoxia (5% CO2 – 95% O2) in presence or absence of Ad for the desired lengths of time (3–24 h).	supplement the media with 10% FBS, non-essential amino acids, antibiotics, and endothelial growth factor.

Upstream tips
Following Ad pre-treatment, cells were exposed to normoxia (5% CO2 – 95% air) or hyperoxia (5% CO2 – 95% O2) in presence or absence of Ad for the desired lengths of time (3–24 h).
Protocol tips
supplement the media with 10% FBS, non-essential amino acids, antibiotics, and endothelial growth factor.

Publication protocol

BPAECs were cultured in MEM supplemented with 10% FBS, non-essential amino acids, antibiotics, and endothelial growth factor. Cells in culture were maintained at 37° C in a humidified environment of 5% CO2 – 95% air. BPAECs, from passages 5 to 20 (75–90% confluence) were used in the experiments.





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