Canine Endothelial Cell Media

Protocol tips

Upstream tips	Protocol tips	Downstream tips
culture the cells on Fibronectin (0.0025% in PBS) coated (2 h/37◦C) cell culture dishes at 37◦C/5% CO2

Upstream tips
culture the cells on Fibronectin (0.0025% in PBS) coated (2 h/37◦C) cell culture dishes at 37◦C/5% CO2

Publication protocol

CnAoECs received as cryopreserved vial containing 500 000 cells were immediately frozen in liquid nitrogen and kept there until use. For further use, cells were thawed at 37°C, resuspended in CECGM (according to the manufacturer's instructions), and cultured on Fibronectin (0.0025% in PBS) coated (2 h/37°C) cell culture dishes at 37°C/5% CO2.





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Manufacturer protocol

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