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CnAOEC plates were precoated with an attachment factor solution (Cell Applications, INC). Cells were cultured at 37°C in a humidified atmosphere in the presence of 5% carbon dioxide and 95% air. |
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Protocol tips |
CnAOEC plates were precoated with an attachment factor solution (Cell Applications, INC). Cells were cultured at 37°C in a humidified atmosphere in the presence of 5% carbon dioxide and 95% air. |
Publication protocol
Canine endothelial cells (CnAOEC) and canine smooth muscle cells (CnAOSMC) from Cell Applications, INC were respectively grown in canine endothelial and canine smooth muscle cell growth mediums (Cell Applications, INC). CnAOEC plates were precoated with an attachment factor solution (Cell Applications, INC). Cells were cultured at 37°C in a humidified atmosphere in the presence of 5% carbon dioxide and 95% air. Medium was changed every 3 days.
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Manufacturer protocol
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