Gibco™ DMEM, high glucose

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	supplemented with 10% fetal bovine serum. L-glutamine (2 mmol/l and antibiotics (1% penicillin/streptomycin). Cells were cultured in a cell incubator providing a humidified environment, with 5% CO2 and 95% air at 37°C.

Protocol tips
supplemented with 10% fetal bovine serum. L-glutamine (2 mmol/l and antibiotics (1% penicillin/streptomycin). Cells were cultured in a cell incubator providing a humidified environment, with 5% CO2 and 95% air at 37°C.

Publication protocol

HAECs were purchased from the Type Culture Collection of the Chinese Academy of Sciences (Shanghai, China) and cultured in Dulbecco's modified Eagle's medium (Gibco; Thermo Fisher Scientific, Inc., Waltham, MA, USA) supplemented with 10% fetal bovine serum (Gibco; Thermo Fisher Scientific, Inc.). L-glutamine (2 mmol/l; Invitrogen; Thermo Fisher Scientific, Inc.) and antibiotics (1% penicillin/streptomycin; Invitrogen; Thermo Fisher Scientific, Inc.). Cells were cultured in a cell incubator providing a humidified environment, with 5% CO2 and 95% air at 37°C.

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