Medium 200
Mammalian cell culture media HAOECv
Publication protocol
Cryopreserved primary human aortic endothelial cells (pHAECs) isolated from a deceased organ donor were recovered from liquid nitrogen and expanded in medium 200 supplemented with low-serum growth supplement (LSGS; Gibco, Life Technologies Corporation, Grand Island, NY) in 1% gelatin-coated 60-mm tissue culture plates (CELLTREAT Scientific Products, Pepperell, MA).
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