Gibco™ DMEM, high glucose

Protocol tips

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	Media with 10% FBS, penicillin (100 U/mL) and streptomycin (100 U/mL)

Protocol tips
Media with 10% FBS, penicillin (100 U/mL) and streptomycin (100 U/mL)

Publication protocol

Human aortic smooth muscle cells (HASMCs) were obtained from Invitrogen and grown at 37 °C in humidified air containing 5% CO2. They were cultured in Dulbecco's modified Eagle's medium (DMEM) containing 10% FBS, penicillin (100 U/mL) and streptomycin (100 U/mL). This medium was replaced with fresh medium every 2 days.

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Manufacturer protocol

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