Gibco™ DMEM, high glucose

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	seeding in multiwell plates coated with a solution of fibronectin and collagen

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seeding in multiwell plates coated with a solution of fibronectin and collagen

Publication protocol

Human bronchial epithelium cells obtained from bronchial biopsy specimens were cultivated under ALI (air liquid interface) conditions. Bronchial epithelial biopsy tissue was dissociated and suspended in bronchial epithelial growth medium (Lonza, Switzerland). After seeding in multiwell plates coated with a solution of fibronectin and collagen, cells were expanded in a flask and then plated on uncoated nucleopore membranes in a 1:1 mixture of bronchial epithelial growth medium and Dulbecco’s modified Eagle medium (Life Technologies, France) applied on the basal side only to establish the ALI. Cells were maintained in culture for 21 days to obtain a differentiated cell population with a mucociliary phenotype.

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