Growth Medium SupplementMix

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	supplemented with 10% fetal bovine serum and 1% penicillin(HyClone; Thermo Fisher Scientific, Waltham, MA, USA).

Protocol tips
supplemented with 10% fetal bovine serum and 1% penicillin(HyClone; Thermo Fisher Scientific, Waltham, MA, USA).

Publication protocol

Human chondrocytes (C-12750) obtained from Promo-Cell (Heidelberg, Germany) were cultured in chondrocyte basal media (C-27111) and a chondrocyte growth media supplement mix (C-39635) with 10% fetal bovine serum and 1% penicillin (HyClone; Thermo Fisher Scientific, Waltham, MA, USA). The samples were sterilized with 70% ethanol for 20 minutes and then rinsed thrice with phosphate-buffered saline. 3-(4,5-Dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) assays were used to determine cell adhesion and proliferation after 1, 3, 5, and 7 days. The cells were seeded at 5,000 cells/cm2 for the proliferation assays. The media was changed every other day. The MTS (CellTiter 96® AQueous One Solution Cell Proliferation Assay, G3581 Promega Corporation, Fitchburg, WI, USA) reagent (1:5 ratio with cell culture media) was added to each well and was incubated for 3 hours on the day of the measurement. Absorbance from each well was measured by a SpectraMax M3 (MT05412; Molecular Devices LLC, Sunnyvale, CA, USA) at 490 nm and a color change from pink to dark brown was seen. Northeastern University Institutional Review board approved the use of human cells during this research.

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Manufacturer protocol

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