Dulbecco’s Modified Eagle’s Medium - high glucose

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	Dermal fibroblasts were cultured in Dulbecco’s modified Eagle medium(DMEM; D6546, Sigma-Aldrich) supplemented with 10% foetal bovine serum (HyClone), 2 mM L-glutamine (G7513, Sigma-Aldrich)and 1% penicillin-streptomycin (P0781, Sigma-Aldrich) in a humidified incubator (37°C, 5% CO2). Cells were passaged once a week at a 1:4 split ratio

Protocol tips
Dermal fibroblasts were cultured in Dulbecco’s modified Eagle medium(DMEM; D6546, Sigma-Aldrich) supplemented with 10% foetal bovine serum (HyClone), 2 mM L-glutamine (G7513, Sigma-Aldrich)and 1% penicillin-streptomycin (P0781, Sigma-Aldrich) in a humidified incubator (37°C, 5% CO2). Cells were passaged once a week at a 1:4 split ratio

Publication protocol

Dermalfibroblasts were cultured in Dulbecco’s modified Eagle medium(DMEM; D6546, Sigma-Aldrich) supplemented with 10% foetal bovine serum (HyClone), 2 mM L-glutamine (G7513, Sigma-Aldrich)and 1% penicillin-streptomycin (P0781, Sigma-Aldrich) in a humidified incubator (37°C, 5% CO2). Cells were passaged once a week at a 1:4 splitratio

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