Fibroblasts growth media 2

Mammalian cell culture media Human Dermal Fibroblasts: HDF

Experiment
Mammalian cell culture media Human Dermal Fibroblasts: HDF
Product
Fibroblasts growth media 2 from PromoCell
Manufacturer
PromoCell

Protocol tips

Protocol tips
NHDF were maintained in fibroblasts growth media 2 (FGM2, Promocell) under the physiological culture conditions (37°C, 5% CO2), and subcultured using DetachKit2-Promocell HEPES BSS (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid buffered saline solution); 0.04% trypsin/0.03% Ethylenediaminetetraacetic acid (EDTA); trypsin neutralizing solution (TNS) containing 0.05% trypsin inhibitor from soybean/0.1% bovine serum albumin).

Publication protocol

NHDF were maintained in fibroblasts growth media 2 (FGM2, Promocell) under the physiological culture conditions (37°C, 5% CO2), and subcultured using DetachKit2-Promocell HEPES BSS (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid buffered saline solution); 0.04% trypsin/0.03% Ethylenediaminetetraacetic acid (EDTA); trypsin neutralizing solution (TNS) containing 0.05% trypsin inhibitor from soybean/0.1% bovine serum albumin).

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Papers

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Paper title
Validation of in vitro assays in three-dimensional human dermal constructs
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Manufacturer protocol

Download the product protocol from PromoCell for Fibroblasts growth media 2 below.

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