Fibroblasts growth media 2

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	NHDF were maintained in fibroblasts growth media 2 (FGM2, Promocell) under the physiological culture conditions (37°C, 5% CO2), and subcultured using DetachKit2-Promocell HEPES BSS (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid buffered saline solution); 0.04% trypsin/0.03% Ethylenediaminetetraacetic acid (EDTA); trypsin neutralizing solution (TNS) containing 0.05% trypsin inhibitor from soybean/0.1% bovine serum albumin).

Protocol tips

NHDF were maintained in fibroblasts growth media 2 (FGM2, Promocell) under the physiological culture conditions (37°C, 5% CO2), and subcultured using DetachKit2-Promocell HEPES BSS (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid buffered saline solution); 0.04% trypsin/0.03% Ethylenediaminetetraacetic acid (EDTA); trypsin neutralizing solution (TNS) containing 0.05% trypsin inhibitor from soybean/0.1% bovine serum albumin).

Publication protocol

NHDF were maintained in fibroblasts growth media 2 (FGM2, Promocell) under the physiological culture conditions (37°C, 5% CO2), and subcultured using DetachKit2-Promocell HEPES BSS (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid buffered saline solution); 0.04% trypsin/0.03% Ethylenediaminetetraacetic acid (EDTA); trypsin neutralizing solution (TNS) containing 0.05% trypsin inhibitor from soybean/0.1% bovine serum albumin).

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